A-Z test index

Cardiovascular risk increases with ApoA1 <1.2 (men) or <1.3 g/L (women) Hypoalphalipoproteinaemia ApoA1 <0.9

Seat the patient 5 minutes before sampling. Fasting sample preferred, but non-fasting acceptable. Patient should follow their normal diet for 3 weeks prior to sampling.

Cardiovascular risk increases with ApoB > 0.9 Dysbetalipoproteinaemia ApoB < 0.5

Seat the patient 5 minutes before sampling. Fasting sample preferred, but non-fasting acceptable. Patient should follow their normal diet for 3 weeks prior to sampling.

Common genotype ApoE3/ E3 CVD risk increases with allele dose of apoE4 Apo E2/E2 associated with remnant (IDL) hyperlipidaemia in the presence of other risk factors ApoE2/E4 and ApoE4/E4 associated with less severe remnant hyperlipidaemias

The assay is specific for the 3 genotypes. Other rarer apoE mutations associated with hypertriglyceridaemia eg ApoELeiden are not detected.

<3

Additional cardiovascular risk marker. To reduce variance needs to be measured twice about 1 month apart.

Results reported for point mutations, exon splicing disorders in the LDLreceptor, apoB exon 27 and PCSK-9 genes

Initial screen of 57 mutations in 3 genes accounting for 50 - 70% of UK mutations performed by iPLEX technology by Genetics Laboratory (Belfast). Patient with evidence of xanthomata or high clinical suspicion of FH receive full sequencing and MPLA analysis if initial screen is negative. Please refer to lipid clinic for pre-screening and cascade testing. Contact Dr Anthony Wierzbicki (x81256) or Dr Martin Crook (x81263). Requires formal consent. Samples processed by Genetics.

Indication: diagnosis of enzyme deficiency. This test is not suitable for subjects with heparin sensitivity, have a history of bleeding diathesis, proliferative retinopathy, CVA, have active rheumatic fever, a peptic ulcer or are taking aspirin. Sample must be received by laboratory within 30 minutes of collection or sent frozen on dry ice Fasted sample required Patient refrained from alcohol in the previous 24 hours and heavy exercise in the previous 48 hours. Please have all cli

CVD risk increases with HCY >12 Levels > 20 may justify MTFHR G677T genotyping

Increased values can be seen during pregnancy, the follicular phase and post-menopause, with a high protein diet and some drugs. Renal function, folate and vitamin B12 status should be investigated on finding a high homocysteine. Sample must be received by laboratory within 30 minutes of collection. Fasting sample required for CVD risk assessment, values lowest in the morning.

Indication: diagnosis of enzyme deficiency in type I and type V Fredrickson hyperlipidaemias. This test is not suitable for subjects with heparin sensitivity, have a history of bleeding diathesis, proliferative retinopathy, CVA, have active rheumatic fever, a peptic ulcer or are taking aspirin. Sample must be received by laboratory within 30 minutes of collection or sent frozen on dry ice Fasted sample required. Patient refrained from alcohol in the previous 24 hours and heavy exe

Cardiovascular risk increases with Lp(a) > 0.3

Additional cardiovascular risk factor whose levels are mostly genetically determined. Indicated for risk assessment in patients with a strong family history of atherosclerosis. Levels are higher in West African and Afro-Caribbean populations. Interfere

Please contact the laboratory to discuss

Indications: Screening for X-linked ichthyosis; confirmation of abetalipoproteinaemia/ NARP syndromes Heparin therapy can lead to alterations in the migration of the lipoproteins, particularly beta-lipoproteins. Fasting sample recommended

Low risk for cardiovascular disease if: Trigylceride < 1.7 Cholesterol < 4 VLDL-C < 0.3 LDL-C < 2 HDL-C (male) > 1.0 HDL-C (female) > 1.2

Indications: diagnosis of lipid disorder especially those associated with hypertriglyceridaemia and classification by Fredrickson WHO criteria. Fasting (12 - 14 hr)

Please contact the laboratory to discuss

Indications: diagnosis of rare lipid disorders (CETP deficiency, LCAT deficiency) Quantification and relative distribution of atherogenic LDL and HDL particles. Reported as cholesterol, triglyceride, apoA1 and apoB contents of fractions consistent with chylomicrons, VLDL, IDL, LDL subfractions 1 - 6 and HDL with interpretation of the profile. Fasting (12 - 14hr)

Aldosterone (pmol/L): Upright 100 - 800, Supine 100 - 450 Aldo/Renin ratio: <80: Conn’s unlikely. >/=200: Conn’s likely. 80-200: Conn’s not excluded.

EDTA Plasma - Process blood samples at room temperature in a non-chilled centrifuge. Plasma should be separated from cells immediately after centrifugation, aliquoted into two 2mL pots (min 500µL in each), and frozen at –20ºC.

Male: 0.10 - 0.80 Female: Non menopausal 0.1 – 0.90 Menopausal 0.1 – 1.60

Take sample to specimen reception immediately after collection. Separate and freeze sample straight away.

At the 3rd day of a regular cycle the AMH concentration of normoovulating women varies from 2.2 - 6.8

Do not use haemolysed samples. Take sample to CSR immediately after collection. Separate and freeze sample straight away